Immune Enhancement of Polysaccharide from Submerged Culture with Phellinus linteus in the Medium Supplemented with Ginseng Extract

摘要

Crude polysaccharide (CP) was fractionated from the submerged cultiu-e (containing both mycelia and culture broth, SO with Phellinus linteus (PL) in mushroom complete medium (MCM) supplemented with ginseng extract (65 deg Bx, GE) to enhance the immuneactivity. PL-GE-15-CP from SC cultivated in MCM supplemented with GE-15% (v/v, a ratio of MCM volume to GE) showed significantly higher macrophage stimulation (1.45 fold of the saline control at 100 mug/mL) than PL-GE-5 and 10-CP with GE-5 and 10%, or PL-CP from SC without GE. The potent intestinal immune system modulating activity through Peyer's patch was also obtained by PL-GE-15-CP (1.46 fold). When PL-GE-15-CP further fractionated on DEAE-Sepharose CL-6B (Cf form), PL-GE-15-CP-n was the significantly higher than others from PL-GE-15-CP or PL-CP on macrophage stimulation, interleukin (XL)-12 production and intestinal immune system modulation (1.54, 3.96 and 1.56 fold, respectively). PL-GE-15-CP-II also had higher anti-metastatic activity against colon 26-M3.1 carcinoma cell (57.3% inhibition of tumor control, 200 mug/mouse) rather than PL-CP-H. This active fraction (PL-GE-15-CP-n) mainly contained neutral sugar (82.45%) and uronic acid (12.99%), and component sugar analysis showed that PL-GE-15-CP-n consisted mainly of uronic acid, Ara, Man, Gal and Glc (molar ratio of 0.52:0.97:0.63:1.00:0.54). Eurthermore, the activity of GE culture was higher compared with culture without GE, indicating that GE helped to enhance the immune activity of P. linteus', also, it is assumed that the polysaccharide plays an important role in immune enhancement.