Effect of broccoli sprouts on cholesterol-lowering and anti-obesity effects in rats fed high fat diet.

摘要

This study was conducted to investigate the cholesterol lowering and anti-obesity effects of an ethanol extract of broccoli sprouts (BS) in rats fed a high fat diet. Male Sprague-Dawley rats weighing 150-155 g, were divided into 6 groups; a normal diet group (ND), a high fat diet group (HFD), a normal diet and low BS (200 mg/kg) treated group (ND-BSL), a normal diet and high BS (400 mg/kg) treated group (ND-BSH), a high fat diet and low BS (200 mg/kg) treated group (HFD-BSL), and a high fat diet and high BS (400 mg/kg) treated group (HFD-BSH). Body wt. gain and mesenteric adipose tissue wt. were increased by the high fat diet, but gradually decreased to the corresponding level of the ND group after administration of BS extract. Liver and epididymal adipose tissue wt. of the HFD group were the highest among the 6 groups, although the difference was not significant. Food intake was lower in the high fat diet groups compared with normal diet groups. Serum alanine transaminase and aspartate transaminase activities, which were elevated by the high fat diet, were significantly decreased after BS administration. Levels of serum total cholesterol, LDL-cholesterol, triglyceride and the atherogenic index tended to decrease in the BS administered groups compared with the HFD group. However, HDL-cholesterol level in serum decreased in the HFD group and markedly increased in the BS administered groups. There were no differences in the contents of serum triglyceride, LDL-cholesterol and HDL-cholesterol between normal diet groups. Levels of total cholesterol and triglyceride in liver and adipose tissues were also lower in BS administered groups than in the HFD group. Activities of heparin-releasable lipoprotein lipase and total-extractable LPL in adipose tissue were increased in the HFD group compared with the BS administered groups, but those of the ND-BSL group and ND-BSH group were similar to the ND group. These results suggest that BS ethanol extract may exert a cholesterol-lowering effect and potentially reduce lipid storage.