Phenolics Enrichment Process from Unripe Apples

摘要

Unripe apples contain significant amount of phenolics with various health benefits. A pilot scale enrichment process of unripe apple phenolics with Viscozyme L extraction and XAD-7 sorption process, based on the total phenolic content (TPC), phenolics content by high-performance liquid chromatography analyses, and antioxidant activities were studied. Antioxidant activities were tested by measuring oxygen radical absorbing capacity (ORAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and ferric-reducing antioxidant power (FRAP). The phenolics-rich final product, apple antioxidant phenolics (AAP) has 771-fold higher TPC and 600-fold higher antioxidant activity of unripe apples. Through XAD-7 sorption AAP showed 52, 87, 70 and 44-fold increases in TPC, ORAC, DPPH and FRAP values, respectively. AAP showed more than 95% stability at the temperature range of 20 to 120 degrees... and pH range of 1.47 to 8.5. Apple phenolics extraction process of Viscozyme L extraction coupled with XAD-7 sorption could be applied to the production of health benefit antioxidants