Isolation and determination of carbendezim residue from wheat grain by matrix solid-phase dispersion and HPLC

摘要

Carbendazim residues were analyzed by column-switching high-performance liquid chromatography (HPLC) with photodiode array detection (PDA). The active ingredient was extracted by matrix solid-phase dispersion (MSPD) from wheat grain on an acidic silica gel column using a methanol-dichloromethane mixture. The recovery rate for fortified samples was 87.3 ± 3.3% with a standard deviation (SD) of 2.9%. The detection limit was 0.02 μg/mL. The method was applied to the determination of carbendazim residues in wheat grain samples from a treated field.