Toxic/Harmful Solvents-Free Technique for HPLC Determination of Six Sulfonamides in Meat

摘要

A simultaneous HPLC determination of six sulfonamides (SAs) [sulfadiazine (SDA), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfadimethoxine (SDM), and sulfaquinoxaline (SQ)] in meat (chicken, beef, and pork) is presented. The sample preparation is carried out by normal-phase matrix solid-phase dispersion (MPSD) with an aqueous ethanol solution. The HPLC determination is performed using a Mightysil RP-4 GP column and an isocratic mobile phase of 2% (v/v) acetic acid solution (pH 2.7, in water)-ethanol (75:25, v/v) with a photodiode array detector. Average recoveries spiked at 0.05-0.5 ppm for each drug are higher than 85% with standard deviations within 10%. In all the processes, no toxic/harmful solvents are used at all.