Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice.

摘要

In this study, the hepatoprotective effects of hyperoside (1), a flavonoid glycoside isolated from Artemisia capillaris, have been examined against carbon tetrachloride (CCl₄)-induced liver injury. Mice were treated intraperitoneally with vehicle or 1 (50, 100, and 200 mg.kg-1) 30 min before and 2 h after CCl₄ (20 micro L.kg-1) injection. Levels of serum aminotransferases were increased 24 h after CCl₄ injection, and these increases were attenuated by 1. Histological analysis showed that 1 prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl₄ treatment, and these changes were reduced by administration of 1. Protein and mRNA expression of tumor necrosis factor- alpha (TNF- alpha ), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl₄ injection. Compound 1 suppressed TNF- alpha , iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that 1 has protective effects against CCl₄-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.