EVALUATION OF BACTERIOPHAGE AV-08 FOR SIMULTANEOUS BIOCONTROL OF SALMONELLA MONTEVIDEO AND ESCHERICHIA COLI O157:H7 IN EXPERIMENTALLY CONTAMINATED CHICKEN SKIN

摘要

The aim of this research was to evaluate the efficacy of bacteriophage Av-08 for reducing Salmonella Montevideo and Escherichia coli O157:H7 presence from chicken skin surface at 4C. Significant reduction (P < 0.05) of S. Montevideo (5.16 log10 colony-forming units [cfu]/4 cm2 chicken skin) and E. coli O157:H7 (4.9 log10 cfu/4 cm2 chicken skin) was achieved. When phage was applied to the bacterial mixture, a reduction of 4.4 and 4.69 log10 cfu/4 cm2 of S. Montevideo and E. coli O157:H7 was obtained on the chicken skin surface, respectively. No significant reduction was observed by buffer application, compared with the control. Phage Av-08 was able to survive on the chicken skin surface at concentrations fluctuating from 7.27 to 8.44 log10 PFU/4 cm2for 6 days. These results demonstrated the ability of phage Av-08 in reducing the presence of S. Montevideo and E. coli O157:H7 from chicken skin surface at 4C.