Optimization of apple pomace based medium and fermentation conditions for pigment production by Chromobacter sp.

摘要

The effect of carbon and nitrogen sources on yield and carotenoid production by Chromobacter sp. [Chromobacterium], a dark red-colour-producing bacteria, was studied in apple pomace-based medium. Apple pomace at a concentration of 20 g/litre showed maximum yield in biomass and carotenoids under the basic medium. The use of maltose (0.3%) in the apple pomace medium gave the highest yield in biomass (6.6 g/litre) and carotenoids (46.3 mg/100 g). Potassium nitrate (0.1%) gave maximum production of biomass (6.5 g/litre) and carotenoids (46.6 mg/100 g), under an optimum pH of 6.0 and an incubation temperature of 35 degrees C. An incubation period of 48 h showed higher yield of biomass and carotenoids..