Indirect enzyme - linked immunosorbent asssay in detection and differentiation of cooked and raw pork from meats of other species

摘要

Indirect enzyme-linked-immunosorbent assay (ELISA) was standardized to identify the cooked and raw (native) muscle antigens of pig and their differentiation from muscle antigens of cattle, buffalo, sheep, goat and chicken. The unabsorbed antiserum (RAP) in rabbits against boiling resistant ethanol precipitable (BE) adrenal antigen of pig was found species specific and revealed promising results in indirect ELISA. It detected all the cooked and raw pork samples fro m field as pig meat and on the basis of cut-off ELISA values (0.14 for cooked, 0.10 for raw) differentiated them from the cooked and raw muscle samples of cattle, buffalo, sheep, goat and chicken. Indirect ELISA proved to be-a sensitive test as it detected adulterant pig meat (cooked/raw) at a minimum level of 1% adulteration in meats of cattle/buffalo/sheep/goat/chicken.