Determination of residual fipronil in chicken egg and muscle by LC-MS/MS

摘要

A simple, sensitive and reliable method was developed and applied to the residue analysis of fipronil in chicken egg and muscle by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Chicken egg and muscle samples were extracted with acetronitrile, then salting out by dehydration with anhydrous magnesium sulfate and sodium chloride. The extracts were purified by the C-18 solid phase extraction cartridge prior to analysis by LC-MS/MS. The matrix-matched calibration curve showed a good linear within the concentration range from 0.01 to 2.00 mu g kg(-1) (r(2) >= 0.999). The average recoveries of fipronil at three spiked levels of 0.01, 0.1 and 1.0 mu g kg(-1) ranged from 79.7% to 98.0%, and the relative standard deviations were less than 8.8%. The decision limit (CCot) and detection capability (CC beta) of fipronil in chicken egg and muscle matrices were all 0.002 mu g kg(-1) and 0.01 pg kg(-1), respectively. The method has also been successfully applied to monitoring fipronil in the real samples. (C) 2016 Elsevier B.V. All rights reserved.