RAPID DETECTION OF FUMONISIN B-1 IN CORN-BASED FOOD BY COMPETITIVE DIRECT DIPSTICK ENZYME IMMUNOASSAY ENZYME-LINKED IMMUNOFILTRATION ASSAY WITH INTEGRATED NEGATIVE CONTROL REACTION

摘要

Membrane-based competitive enzyme immunoassays (EIA) in dipstick. and immunofiltration (ELIFA) format for the detection of fumonisin B-1 (FB1) were developed. A nylon membrane was coated with anti-FB1 antibodies and with anti-horseradish peroxidase (HRP) antibodies. A FB1-HRP conjugate was used both as the labeled antigen for competitive assay of FB1 and for noncompetitive binding to the anti-HRP antibodies (negative control). Both tests had visual detection Limits for FB1 in the range of 7.5-10 ng/mL (buffer solutions) and 40-60 ng/g (corn-based food samples). The tests were applied to corn-based food samples, and the results were compared with those of microtiter plate EIA and high-performance Liquid chromatography. With assay times of 60 min (dipsticks) and 10 min (ELIFA), these rapid tests should be useful to qualitatively detect FB1 in corn-based foods.