HPLC and mass spectrometric analysis of carotenoids from mango.

摘要

beta-Carotene (all-trans), beta-cryptoxanthin (all-trans and cis), zeaxanthin (all-trans), luteoxanthin isomers, violaxanthin (all-trans and cis) and neoxanthin (all-trans and cis) were identified in mango by means of HPLC data (retention time, co-injection, and chemical reactions). Mass spectrometry (molecular ion, fragmentation pattern, m/z relative intensity) confirmed the identity of these carotenoids (not the geometric form); additionally, lutein and mutatochrome were detected when a large amount of sample was utilized. The quantitative composition was determined by HPLC, Sudan I being used as internal standard. all-trans-Violaxanthin (21.1±2.9μg/g), all-trans-beta-carotene (15.1±1.5μg/g) and a cis-violaxanthin (10.1±0.2μg/g), tentativelyidentified as 9-cis, were the principal carotenoids.