Novel defective interfering DNAs associated with ageratum yellow vein geminivirus infection of Ageratum conyzoides.

摘要

To search for additional genomic components which may be necessary for ageratum yellow vein bigeminivirus (AYVV) infection of A. conyzoides, naturally-infected A. conyzoides plants were collected from wastelands in Singapore. Virus-specific supercoiled DNA was purified by CsCl centrifugation and clones containing viral DNA inserts were identified by colony hybridization. The supercoiled DNA did not contain detectable levels of a DNA B component, but novel defective DNAs associated with infected A.conyzoides were identified. The defective DNA had a significant effect on AYVV DNA accumulation and symptom development when reintroduced into Nicotiana benthamiana by inoculation.