Inhibitory activity of phosphorylated chitooligosaccharides on the formation of calcium phosphate

摘要

Chitooligosaccharides (COSs) were successfully prepared using an ultrafiltration membrane bioreactor system,and named as COS-I (10-5 kDa),COS-II (5-3 kDa),COS-III (3-1 kDa),and COS-IV (below 1 kDa),respectively.In addition,their phosphorylated derivatives were prepared by a P_2O_5 in methanesulfonic acid solution,and inhibitory activity on the formation of insoluble calcium phosphate of phosphorylated chitooligosaccharides.Phosphorylated COS-IV exhibited the highest inhibitory activity of calcium phosphate precipitation among tested chitooligosaccharides.Its inhibitory activity,especially at the concentration more than 4 mg/ml,was similar to that of CPP,which is widely used as a calcium fortifying agent increasing calcium absorbability.Therefore,phosphorylated chitooligosaccharides may be potential inhibitors of calcium phosphate precipitation.