Ultrasound-Assisted Extraction, Preliminary Characterization, and Antioxidant Activity of a Novel Water-Soluble Polysaccharide from Lotus (Nelumbo nucifera Gaertn.) Seeds

摘要

Ultrasound-assisted extraction was employed to extract polysaccharides from lotus (Nelumbo nucifera Gaertn.) seeds. Response Surface Methodology was used to determine the optimal extraction conditions of ultrasound power, extraction time, and extraction temperature at 406 W, 7.7 min, and 94°C, respectively. The crude extract was successively purified by chromatography, yielding a major polysaccharide fraction termed LSPS-1. It is a heteropolysaccharide, containing rhamnose, arabinose, glucose, and galactose with a molecular weight of 4484 Da and an α-dominating configuration in pyranose form sugar. Preliminary antioxidant activity test in vitro showed LSPS-1 could potentialize the scavenging effect on hydroxyl radicals in a dose dependent manner.View full textDownload full textKeywordsantioxidant activities, characterization, extraction, lotus seeds, polysaccharideRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/01496395.2012.665118