Assessing the effects of Awba dam sediment (Nigeria) on the steroidogenesis of H295R cells using different extraction methods

摘要

In the present study, H295R human cells were used to investigate the endocrine disruptor potential of three different sediments extracts taken from a Nigerian tropical freshwater dam (Awba Dam), using three extraction methods that allowed a selective consideration of contaminants based on their binding affinity, which is mainly driven by polarity, to sediment particles. After exposure to different concentration of each extract, H295R cells were evaluated for the expression profiles of 10 steroidogenic enzyme genes and estradiol (E2) and testosterone (T) levels. Our results showed a comparable concentrated-related increase in the expression of 17 beta-hsd1, 3 beta-hsd2 and cyp21 in cells treated with the polar and non-polar extracts. The star, hmgr, cyp11b2 and 17 beta-hsd4 were slightly decreased, in an apparent concentration-specific manner, after treatment with the polar extract and decreased in the non-polar treatment. The cyp11a and cyp17 showed an opposite trend in the polar and non-polar treatments. E2 was significantly higher in cell treated with the non-polar extract. Elutriate exposure produced less pronounced variation in mRNA and hormones levels. Overall the extract with non-polar compounds produced the most severe effects in H295R cells. Thus, direct ingestion of detritus and mud from fishes and other benthonic organisms represent possible transfer of contaminants in the trophic web, and mainly account for alteration of the endocrine system previously observed in fish from the same study site. (c) 2018 Elsevier B.V. All rights reserved.