A TESTICULAR ANTIGEN ABERRANTLY EXPRESSED IN HUMAN CANCERS DETECTED BY AUTOLOGOUS ANTIBODY SCREENING

摘要

Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens, We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets, One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues, Reverse transcription-PCR analysis showed NY-ESO-I mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma, NY ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein, The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion, These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination. [References: 33]