Analysis of Dynamic Processes in Single-Cell Electroporation and Their Effects on Parameter Selection Based on the Finite-Element Model

摘要

Pulsed electric fields have recently been the focus of considerable attention because of their potential application in biomedicine. However, their practical clinical applications are limited by poor understanding of the interaction mechanism between pulsed electric fields and cells, particularly in the process of electroporation and its effect on parameter selection. This paper established a multishelled dielectric model based on finite elements to simulate and analyze the processes involved in electroporation. In particular, the processes include the dynamic development of the pore radius and electroporation region: the distribution of recoverable, nonrecoverable, and nonelectroporation areas on the cell; and the influence of pulse parameters on varying degrees of electroporation. Results showed that membrane conductivity, pore density, transmembrane potential, and distribution of pore radii are functions of time and position on the cell. The electroporation areas were divided into recoverable, nonrecoverable, and no-electroporation pores. For 10~mu text{s} , 1.5-kV/cm pulse was observed in the regions exposed to sufficiently high transmembrane voltage (1 V), electroporation occurred, membrane conductivity and pore density (up to 10^{mathrm {16}}/text{m}^{mathrm {2}}) rapidly increased with time, and electroporation areas increased gradually and were mainly distributed in the range 0°–70° (recoverable pore [0°, 35°], nonrecoverable pore [35°, 70°], and no-electroporation pore [70°, 90°]). Electric field strength was the major factor that induced electroporation, particularly in the recoverable pore, but it had minimal effect on pore expansion. However, pulse duration affects the nonrecoverable pore, such that the high-intensity wide pulse is more useful in the field of irreversible electroporation. The high-intensity short pulse can increase permeability and maintain cell viability.