Surface Plasmon Resonance Imaging Analysis of Protein-Receptor Binding in Supported Membrane Arrays on Gold Substrates with Calcinated Silicate Films

摘要

Surface plasmon resonance imaging (SPRi) is a powerful tool for label-free and real-time studies of biological interactions,with the potential for high-throughput analysis in crucial areas such as pharmaceutical screening and proteomics.The surface functional-ization chemistry for gold SPR substrates is primarily based on alkanethiol SAMs,and involves soft lithography,spotting,microcontact printing,and microfluidic patterning.