Hydrolysis of β-casein(193-209) Fragment by Whole Cells and Fractions of lactobacillus casei an Lactococcus lactis

摘要

Whole cells and fractions of Lactococcus lactis subsp. lac- tis IFPL 359 and Lactobacillus casei subsp. casie IFPL731 wer studied. Hydrolysis productes were separated by re- versed-phase, high-performance liquid chromatogaphy (RP- HPLC). Under conditions, pH 5.2 and 3 /100 NaCl, L. casei IFPL 731 was more active in hydrolysis of the β-casein (f193-209) peptide than was L. lactis IFPL 359. This hydrolyzing activ- ity was attributed for L. casei IFPL 731 by the cell-wall pro- teinase.