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Reserch Note: Effects of Iron and Selenium on the Production of Catalse, Superoxide Dismutase, and Listeriolysin O in Listeria monocytogenes

机译:研究注:铁和硒对单核细胞增生性李斯特菌中过氧化氢酶,超氧化物歧化酶和李斯特菌溶血素O的产生

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摘要

Listeria monocytogenes 19112, Scott A, and 10403S were grown in tryptic soy broth (TSB) and TSB supplemented with 25 to 100 μg/ml of iron (Fe) and 0.5 to 2.5 μg/ml selenium (Se) to examine the effects on catalase (CA), superoxide dismutase (SOD), and listeriolysin O (LLO) activities. Growth in TSB supplemented with Fe resulted in significant increases in CA, SOD, and LLO activities in all three strains when compared to growth in TSB. The addition of 0.5 μg/ml Se to TSB resulted In significantly higher CA and LLO activities in L. Monocytogenes 19112 but showed no effect on Scott A or 10403S. These Results suggest that Fe plays a role in increasing the activities of CA, SOD, and LLO.
机译:在添加了25至100μg/ ml铁(Fe)和0.5至2.5μg/ ml硒(Se)的胰蛋白酶大豆肉汤(TSB)和TSB中生长单核细胞增生性李斯特菌19112,Scott A和10403S,以检查对过氧化氢酶的影响(CA),超氧化物歧化酶(SOD)和李斯特菌溶血素O(LLO)活性。与TSB的生长相比,添加Fe的TSB的生长导致所有三种菌株的CA,SOD和LLO活性显着增加。向TSB中添加0.5μg/ ml Se会导致单核细胞增生李斯特氏菌19112中的CA和LLO活性显着提高,但对Scott A或10403S没有影响。这些结果表明,Fe在增加CA,SOD和LLO的活性中发挥了作用。

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