Preparation of high purity triacylglycerol of punicic acid from pomegranate seeds

摘要

Pomegranate seeds are rich in punicic acid, a x-5 fatty acid which renders highly beneficial effects on human health. However, due to very unstable nature of punicic acid, its quantification becomes difficult. In the present study, we propose a simple methodology of preparation of a sample of Triacylglycerol of punicic acid (Pu-Pu-Pu, > 97% purity) from pomegranate seeds using RP-HPLC. Fraction 6 from HPLC of PSO was found to possess a high content of Pu. Further identification and characterization of the sample have been carried out using GC-MS and NMR. The sample has been found to be stable for 90 days at 220 ℃.