Optimization of ultrasound-assisted extraction of antioxidative phenolic compounds from Deverra scoparia Coss. & Durieu (flowers) using response surface methodology

摘要

The current study aims to optimize the ultrasound-assisted extraction of Deverra scoparia Coss. & Durieu (flowers) antioxidative phenolic compounds using response surface methodology (RSM). A Box-Behnken design model has been conducted to investigate the effect of three factors, namely the extraction period, temperature, and sample-to-methanol ratio, on five responses, namely: extraction yield (Y_%), Total Phenolic Content (TPC), Flavonoids Content (FC) and two antioxidant tests DPPH˙ and FRAP. The used model, proposed to run the extraction at 50°C for 55 min duration with a sample-to-methanol ratio (wt/vol) of 1:70 g/mL. Under optimum conditions (proposed), the experimental values of extraction yield, TPC, FC, DPPH-IC_(50), and FRAP-IC_(50) were 10.785%, 922.107 mg GAE/100 g dry matter, 255.634 mg QE/100 g dry matter, 0.113 g/L and 0.066 g/L, respectively. The experimental values were in agreement with the predicted values, indicating the capability of the model and succeed of RSM in optimizing the extraction conditions.