Simultaneous determination of chlorogenic acids in green coffee bean extracts with effective relative response factors

摘要

Chlorogenic acids contribute great to the quality of green coffee bean extracts. In this article, the qualitative and quantitative analysis of multi-components by single-marker was proposed to simultaneously determine the contents of seven chlorogenic acids (3-CQA, 4-CQA, 5-CQA, 5-FQA, 3,4-diCQA, 3,5-diCQA, and 4,5-diCQA) in 14 samples of green coffee bean extract. 5-CQA was the single-mark to calculate the contents of chlorogenic acids with relative response factors. The reliability and stability of the relative response factors of chlorogenic acids were studied. Taguchi's method with orthogonal array of L16 was applied in design of experiment and the minitab v16 software was used to analyze the relative response factors. The fluctuation and stability of the relative response factors were determined by analysis of variance statistical approach. The impact and optimization of columns and instruments were also studied by Taguchi Analysis. The combination of Venusil MP column and WUFENG instrument was chosen to determine the chlorogenic acids. As a comparison, the quantitative analysis of multi-components by multiple standards also had been done in this article. The compared results showed that there were no great differences in the contents of chlorogenic acids determined by quantitative analysis of multi-components by multiple standards and quantitative analysis of multi-components by single-marker with relative response factors obtained in this work.