Determination of Oxytetracycline in Meat and Fish Using Metal Chelate Resin for Clean-up

摘要

A rapid and reliable quantitative screening procedure was developed for analysis of oxy- tetracycline (OTC) in animal-derived foods. The procedure involves clean-up using metal chelate (MC) resin and on-line confirmation. Samples were homogenized in 0.25/100 metaphosphoric acid methanol-acetonitrile (6: 2: 2). OTC was selectively extracted onto MC resin preloaded with Cu~2+ and determined by HPLC with fluorescence detection using a Mg~2+ -containing mobile phase.