Comparison of TaqMan~TM Salmonella Amplification/Detection Kit with Standard Culture Procedure for Detection Of Salmonella in Meat Samples

摘要

We evaluated the TaqMan PCR Salmonella amplification/detection kit (PE Applied Biosys- tems) for rapid detection of Salmonella from a variety of meat samples. This system uses the 5' nuclease activity of Taq DNA polymerase, which digests and internal fluorogenic probe to monitor the amplification of the target gene. The detection sensitivity of the kit, using 2 kinds of DNA extraction protocols, was compared with that obtained with 4 protocols of official culture methods.