Physicochemical and functional properties of silver carp (Hypophthalmichthys molitrix) myofibrillar protein glycated with konjac oligo-glucomannan

摘要

Konjac oligo-glucomannan (KOG) is a new-type polysaccharide with abundant hydroxyl groups and a small quantity of branches. Myofibrillar protein (Mf) from silver carp (Hypophthalmichthys molitrix) was incubated with KOG for 0-96 hat 50 degrees C and 75% relative humidity to obtain glycoconjugates in different periods of Maillard reaction. Physicochemical properties, including SDS-PAGE, sugars bound to Mf, available amino acids and FT-IR property all indicated that glycation occurred successfully. In addition, activity of Ca2+-ATPase decreased markedly due to thermal denaturation and glycation of Mf. Functional properties of Maillard reaction productions (MRPs), such as solubility, heat stability and emulsion properties were all superior to Mf. Solubility and emulsion properties reached the highest value at 8-12 h of the reaction, and then decreased severely. However, heat stability decreased at 2-5 h of glycation, and then increased significantly. In conclusion, KOG is an excellent sugar donator to improve functional properties of Mf by glycation. (C) 2017 Elsevier Ltd. All rights reserved.