Purification and identification of Capulin (Prunus serotina Ehrh) anthocyanins

摘要

Capulin (Prunus serotina Ehrh) anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using acetone, the aqueous acetone extract partitioned with chloroform and purified in a C-18 solid-phase cartridge. pH-differential and bisulfite bleaching mehods were used to determine monomeric anthocyanin content and polymeric color. Pigments were identified by spectral analyses, HPLC and mass spectroscopy.