Simultaneous determination of phenolic compounds and tocopherols in virgin olive oil using HPLC and UV detection

摘要

A simple procedure is described for the simultaneous extraction and HPLC determination of phenols and tocopherols in virgin olive oil. The extraction was carried out using methanol and an isopropanol- methanol mixture. Separation was achieved on a reversed phase C_18 column with acetic acid/water-methanol-acetonitrile-isopropanol mixture under gradient elution.