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Phytochemical profile, mineral content, and antioxidant activity of Olea europaea L. cv. Cornezuelo table olives. Influence of in vitro simulated gastrointestinal digestion

机译:植物化学型材,矿物质含量和Olea Europaea L.CV的抗氧化活性。 Cornezuelo表橄榄。体外模拟胃肠消化的影响

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The main goals of this study were to determine the phenolic composition and antioxidant activity of table olives from Olea europaea L. cv. Cornezuelo, as well as the effect caused by a simulated in vitro digestion to evaluate compounds bioavailability. High-performance liquid chromatography with diode-array and mass spectrometry detection (HPLC-DAD-MSn) was used to evaluate the phytochemical profile, whereas conventional spectrophotometric methods (ABTS center dot(+) and DPPH) were used to determine the antioxidant activity. The mineral content was also quantified by inductively coupled plasma - mass spectrometry.Thirty compounds were identified, mainly polyphenols, quantifying the major compounds by HPLC-DAD. After the simulated digestion, the phenolic content suffered an important decrease - more than 50% - reaching losses of up to 75% for oleuropein and comselogoside isomers. This decrease also resulted in a loss of antioxidant activity, observing significant differences for all parameters. However, the analyzed extracts still retained considerable antioxidant potential.
机译:本研究的主要目标是从Olea Europaea L.CV中确定表橄榄的酚醛组合物和抗氧化活性。 Cornezuelo,以及模拟体外消化引起的效果,以评估化合物生物利用度。使用二极管阵列和质谱检测(HPLC-DAD-MSN)的高性能液相色谱法评估植物化学曲线,而常规分光光度法(ABTS中心点(+)和DPPH)用于确定抗氧化活性。通过电感耦合等离子体质谱法量化矿物质含量。鉴定了三种化合物,主要是多酚,通过HPLC-DAD定量主要化合物。在模拟消化后,酚醛含量遭受重要降低 - 大于50%至50% - 达到油肺蛋白素和康糖苷异构体的损失高达75%。这种降低也导致抗氧化活性的丧失,观察所有参数的显着差异。然而,分析的提取物仍然保持相当大的抗氧化潜力。

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