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Phytochemical profile, mineral content, and antioxidant activity of Olea europaea L. cv. Cornezuelo table olives. Influence of in vitro simulated gastrointestinal digestion

机译:油橄榄的植物化学特性,矿物质含量和抗氧化活性Cornezuelo食用橄榄。体外模拟胃肠消化的影响

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The main goals of this study were to determine the phenolic composition and antioxidant activity of table olives from Olea europaea L. cv. Cornezuelo, as well as the effect caused by a simulated in vitro digestion to evaluate compounds bioavailability. High-performance liquid chromatography with diode-array and mass spectrometry detection (HPLC-DAD-MSn) was used to evaluate the phytochemical profile, whereas conventional spectrophotometric methods (ABTS center dot(+) and DPPH) were used to determine the antioxidant activity. The mineral content was also quantified by inductively coupled plasma - mass spectrometry.Thirty compounds were identified, mainly polyphenols, quantifying the major compounds by HPLC-DAD. After the simulated digestion, the phenolic content suffered an important decrease - more than 50% - reaching losses of up to 75% for oleuropein and comselogoside isomers. This decrease also resulted in a loss of antioxidant activity, observing significant differences for all parameters. However, the analyzed extracts still retained considerable antioxidant potential.
机译:这项研究的主要目的是确定油橄榄(Olea europaea L. cv)食用橄榄的酚类成分和抗氧化活性。 Cornezuelo以及模拟体外消化所产生的影响,以评估化合物的生物利用度。使用具有二极管阵列和质谱检测功能的高效液相色谱(HPLC-DAD-MSn)来评估植物化学特征,而使用常规分光光度法(ABTS中心点(+)和DPPH)来确定抗氧化活性。矿物含量也通过电感耦合等离子体质谱法进行了定量。鉴定了30种化合物,主要是多酚,通过HPLC-DAD定量了主要化合物。经过模拟消化后,橄榄苦苷和Comselogosideside异构体的酚含量显着下降-超过50%-损失高达75%。该降低还导致抗氧化剂活性的损失,观察到所有参数的显着差异。但是,分析的提取物仍然保留了相当大的抗氧化潜力。

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