Simple validated method for simultaneous determination of deoxynivalenol, nivalenol, and their 3-β-D-glucosides in baby formula and Korean rice wine via HPLC-UV with immunoaffinity cleanup

摘要

A simple and reliable method for the simultaneous determination of major type B trichothecene mycotoxins, deoxynivalenol (DON) and nivalenol (NIV), along with their 3--d-glucosides (DON-3-glucoside (DON3G) and NIV-3-glucoside (NIV3G)) in baby formula and Korean rice wine was validated in the present study. The method was based on immunoaffinity cleanup followed by analysis using an HPLC-UV technique. The method was validated in-house for two matrices as follows: linearity (R-20.99) was established in the range of 20-1000g kg(-1); accuracy (expressed as recovery) ranged from 78.7 to 106.5% for all the analytes; good intermediate precision (relative standard deviation 12%), and adequate detection and quantitation limits ( 4.4 and 13.3g kg(-1), respectively) were achieved. Furthermore, the estimated measurement expanded uncertainty was determined to be 4-24%. The validated method was successfully applied to the analysis of 31 baby formulas and Korean rice wines marketed in Korea.