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Proteolysis, texture and colour of a raw goat milk cheese throughout the maturation

机译:整个成熟过程中生山羊奶干酪的蛋白水解,质地和颜色

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Changes on chemical and textural parameters were studied throughout maturation of PDO Ibores cheese. NCN/TN (non-casein nitrogen/total nitrogen) values were significantly modified (P 0.001) throughout maturation, and significantly, lower values were observed at day 1 than at day 30, 60 and 90. Non-protein nitrogen/total nitrogen (NPN/TN) was not significantly changed during the ripening process (P 0.05). Therefore, proteolysis extent (casein degradation and ‘ripening depth’) took place at initial stage and was limited throughout Ibores cheese maturation. In addition, since casein nitrogen decreased during the first month of ripening without a simultaneous increase in NPN/TN, this likely indicates that the large fragments of caseins liberated during this period are not further degraded into smaller products. Primary proteolysis (resulting from the action of endoproteases on caseins) is thus far more important than secondary proteolysis during this period. Moreover, secondary proteolysis remains limited along the 90 days. Polypeptide nitrogen significantly increased at day 30 from casein degradation compared to day 1, while free amino acids (FAA) content significantly increased during maturation process. In addition, hardness and adhesiveness values significantly increased, but cohesiveness and springiness significantly decreased up to day 60. Variables such as dry matter, NCN/TN and polypeptide nitrogen showed high correlations with textural parameters. Principal component analysis (PCA) of variables divided the Ibores cheeses according to their ripening: early, middle or late ripening.
机译:在PDO Ibores奶酪的整个成熟过程中研究了化学和质地参数的变化。在整个成熟过程中,NCN / TN(非酪蛋白氮/总氮)值均发生了显着变化(P <0.001),并且与第30、60和90天相比,第1天的NCN / TN值显着降低。非蛋白质氮/总氮(NPN / TN)在成熟过程中没有显着变化(P> 0.05)。因此,蛋白水解程度(酪蛋白降解和“成熟深度”)发生在初始阶段,并且在整个Ibores奶酪成熟过程中受到限制。另外,由于酪蛋白氮在成熟的第一个月减少,而NPN / TN却没有同时增加,因此这可能表明在此期间释放的酪蛋白大片段不会进一步降解成较小的产物。因此,在此期间,一级蛋白水解(由于内切蛋白酶对酪蛋白的作用而产生)比二级蛋白水解重要得多。此外,在90天内,二次蛋白水解作用仍然有限。与第1天相比,酪蛋白降解的第30天多肽氮显着增加,而在成熟过程中游离氨基酸(FAA)含量显着增加。此外,直到第60天,硬度和粘合性值都显着增加,但内聚性和弹性却显着降低。诸如干物质,NCN / TN和多肽氮等变量显示出与质地参数高度相关。变量的主成分分析(PCA)根据Ibores奶酪的成熟程度将其划分为:早熟,中熟或晚熟。

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