COMPOSITION AND CONTENT ANALYSIS OF SUGARS AND ORGANIC ACIDS FOR 45 GRAPE CULTIVARS FROM NORTHEAST REGION OF CHINA

摘要

The qualitative and quantitative analysis of sugars and acids of grape cultivars from northeast region of China wascarried out for quality evaluation and variety improvement of grape. Analysis of major sugars and organic acids for 45 grapeberries was carried out using High Performance Liquid Chromatography (HPLC). The result showed that glucose andfructose were the major sugars, beside that, some grape cultivars also contained sucrose. The quantity of glucose andfructose was almost equal in most of grape berries. A significant positive correlation existed between them, glucose contentranged from 53.24 mg/ml to124.18mg/ml and fructose content ranged from 48.39 mg/ml to118.84 mg/ml. Tartaric acid,malic acid, citric acid and oxalic acid were organic acids and tartaric acid was the main constituent in most grape berries andits concentration was higher than the other organic acids. However, in some grape cultivars, malic acid and citric acid weretwo highest organic acids while oxalic acid content was the lowest and even in some cultivars it could not be detected.Tartaric acid ranged from 1.28mg/ml to 6.82 mg/ml, malic acid ranged from 0.09mg/ml to 3.95 mg/ml, citric acid rangedfrom 0.08mg/ml to 4.43 mg/ml, oxalic acid ranged from0 mg/ml to 0.370 mg/ml. Thirty-four grape cultivars out of 45cultivars accounted more than 50% tartic acid of the total organic acid contents. However, in cultivars Bixiang Wuhe andShennong Jinhuanghou citric acid was the main organic acid. Malic acid and citric acid were significantly positively relatedwith total acid. In 43 grape cultivars, the soluble sugars were glucose and fructose. Besides glucose and fructose, sucrosewas also observed in cultivars of LN33 and Cayuga white.