Inhibitory Activities of Lactic Acid Bacteria against Multi-Drug Resistant Uropathogenic &i&Staphylococcus saprophyticus&/i& Isolated from Symptomatic Women in Lagos, Nigeria

摘要

The uropathogenic Staphylococcus saprophyticus is reported severally to be resistant to the drugs often used empirically for treatment of urinary tract infections (UTIs). Their ability to exhibit resistance to multiple drugs is a great de al of threat to successes recorded in the management of UTIs cause d by this pathogen. Lactic acid bacteria (LAB) have been demonstrated to exhibit an timicrobial activities but studies about their prospect against multi -drug resistant S. saprophyticus are quite few. This study therefore investigated acti vities of LAB against the multi-drug resistant S . saprophyticus recover ed fro m urine samples of symptomatic women. The three differ ent species of LAB ( Lactobacillus fermentum BTA 62, Lactobacillus johnsonii BTA 86 and Weis sella confusa BTA 40) previously isolated and identified by 16S rRNA s e quencing were selected based on their history of antimicrobial activities. Their m etabolites were employed in the antagonistic assays against six (6) mu lti-drug resista nt test pathogens recovered fro m urine samples of symptomatic, non-pregnant women attending clinics in Lagos, Nigeria and the control ( S . saprophyticus subs bovis strain DSM 18669) following standard procedures. The pathogens showed resistances to almost all the antibiotics except levofloxaci n, ciprofloxacin, imipenem while the control showed resistance to thr ee. The LAB, L. fermentum inhibited five (83.3%) of the pathogens with zone diameter of 12 - 17 mm, followed by W . confusa inhibiting three (50%) with 15 - 17 mm. Lactobacillus johnsonii on the other hand, inhibited a pathogen and the control with zones of 13 mm and 14 mm respectively. In conclusion, the extracted metabolites of LAB inhibited the growth of multi-drug resistant clinical isolates of uropathogenic S . saprophyticus and may therefore be potent alternatives to antibiotics.