EVALUATION OF THE INHIBITORY ACTIVITY OF LACTIC ACID BACTERIA AGAINST SALMONELLA SPP. AND LISTERIA MONOCYTOGENES ISOLATED FROM CHICKEN CARCASSES

摘要

Cells of lactic acid bacteria (LAB) isolated from the neck skin of poultry carcasses were added to BHI broth along with cells of Salmonella spp. and Listeria monocytogenes in order to determine their antagonistic activity against the pathogens during storage at 7°C. There was a statistically significant reduction (PSO.OS) in Salmonella population at the 5~(th) day of storage that varied from 0.41 to 1.12 log cfu/ml, The protective effect of LAB isolates was also statistically significant (PS0.05) for Listeria monocytogenes where the reduction of its population at the 5~(th) day of storage varied from 0.77 to 1.48 log cfu/ml. The LAB strain (Lactobacillus salivarius), with the best inhibitory activity, was chosen to examine its action against the same pathogens {Salmonella spp and Listeria monocytogenes) on chicken skin. On chicken skin, the growth reduction at the 6th day of storage caused by L. salivarius was lower and did not exceed 0.54 log cfu/cm~2 for Salmonella spp. and 0.71 log cfu/cm~2 for Listeria monocytogenes. The results of the experiments suggest that L. salivarius has a potential to be used as protective culture, in combination with other microbial hurdles, to improve the safety and extent the shelf life of chicken products.