首页> 外文期刊>Journal of the Chilean Chemical Society >HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
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HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA

机译:液相色谱-质谱法快速分离和鉴定槲皮中多酚类物质的高效离心色谱法(HSCCC)

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High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation mass spectrometric detection (ESI-MS) was used to carry out a comprehensive characterisation of phenolic compounds from fruits and leaves of this plant. The main phenolics were the quercetin diglycoside rutin and the triglycoside manghaslin. Nine minor flavonoids were tentatively identified as kaempferol, quercetin, isorhamnetin and methoxymyricetin glycosides. HSCCC combined with other chromatographic methods allowed the isolation of the main phenolics from the plant. HPLC-DAD-MS analysis shows that the main phenolic compounds in V. quercifolia are similar to those described for V. pubescens but differs in the identity of the minor constituents.
机译:高速离心逆流色谱法(HSCCC)已成功地用于从秘鲁Caricaceae Vasconcellea quercifolia A. St.-Hil中分离出主要极性酚类。带有二极管阵列的高效液相色谱(HPLC-DAD)和电喷雾电离质谱检测(ESI-MS)用于对该植物果实和叶片中的酚类化合物进行全面表征。主要酚类是槲皮素二糖苷芦丁和三糖苷甘草肽。初步鉴定出九种次要类黄酮为山奈酚,槲皮素,异鼠李素和甲氧基杨梅甙。 HSCCC与其他色谱方法相结合可从工厂中分离出主要酚类。 HPLC-DAD-MS分析表明,槲皮草中的主要酚类化合物与耻骨弧菌相似,但次要成分不同。

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