A Preparation Method of β-Carotene Reference Standard from β-Carotene Food Additive Based on High Speed Countercurrent Chromatography (HSCCC)

摘要

The paper provided a simple method for preparing the β-carotene reference standard from a β-carotene food additive (96%) based on high speed countercurrent chromatography (HSCCC). The obtained material was qualitatively detected by IR, NMR, MS, the results showed the material was purified β-carotene, the purity of obtained β-carotene reference standard can be as high as 99%. The prepared reference standard can be used for quality control of related health food. The provided method is simple, universal, cheap, and the sample preparation can be enlarged to g grade.