Preparation of Mevinolinic Acid from Monascus purpureus Using High-Speed Countercurrent Chromatography (HSCCC)

摘要

High-speed countercurrent chromatography (HSCCC) was applied for the separation of a crude Monascus purpureus extract. The separation was carried out using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1/1/1/1, v/v) at a flow rate of 1.0 mL/min. From 250 mg of the alkaline treated extract the method yielded 40 mg of mevinolinic acid with a purity of 99% in each separation. The product was confirmed as mevinolinic acid by electrospray ionization multiple mass spectrometry (ESI-MS) and NMR analysis.