An In vitro Evaluation of an Ageratum conyzoides Linn. Extract on the on the Labeling of Sanguineous Cells and Proteins with 99m Technetium

摘要

Ageratum conysoides is a plant with analgesic, antibacterial, anti-inflammatory, depurative, febrifuge, stimulant and vulnerary properties. Blood cells (BC) labeling with 99mTc(technetium-99m) has progressed around the world. The influence of drugs (natural and synthetic) on the labeling of blood elements with 99mTc has been reported. The purpose of this study was to examine the effect of an A. conysoides extract on the radiolabeling of blood elements with 99mTc. Extract (0.1g/mL) was prepared with NaCl (0.9%) and diluted in five different concentrations 100%; 50%; 25%; 12.5% and 6.25% v/v. Blood was withdrawn and it was incubated with the referred extract for 1hour. Elapsed this period of time stannous chloride was added for more one hour followed by the addition of 99mTc. Plasma (P) and (BC) were separated P and C were precipitated with5% trichloroacetic acid (TCA) and soluble (SF) and insoluble (IF) fractions were obtained. The percentage of radioactivity (%ATI) was determined. Related to the results obtained it was noticed that the extract was capable of altering the efficiency of radiolabeling in Cells to the concentrations of 100% (from 96.86?1.80 to 52.84?2.26); 50% (from 96.86?1.80 to 58.05?11.63); 25% (from 96.86?1.80 to 81.98?8.57) and 12.5% (from 96.86?1.80 to 88.83?8.57); in the IF-C to the concentrations of 100% (79.97?9.87 to 15.85 ? 7.06); 50% (from 79.97?9.87 to 17.54?8.00); 25% (from 79.97?9.87 to 31.73?9.04) and 12.5% (79.97?9.87 to 48.74?9.90) and in the IF-P to the concentrations of 100% (from 89.04?5.41 to 41.04?8.67) and 50% (from 89.04?5.41 to 35.40?7.38). Due to the results found we can suggest that refereed extract was capable of altering the radiolabeling of blood elements with 99mTc probably could be explained by the generation of reactive Oxygen species with oxidant properties, which might be altering the stabilizing of the red blood cell membrane as the radiolabeling of blood elements.