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Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults

机译:年轻人唾液中印尼罗伊氏乳杆菌的分离与鉴定

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Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415?and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.? Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
机译:背景:生物膜参与多种微生物感染,包括龋齿和牙周炎。益生菌的使用已经成为一种有前途的预防和治疗方式,可用来对抗口腔中与生物膜相关的疾病。益生菌罗伊氏乳杆菌已被证明可以减少口腔内的牙龈炎和牙菌斑指数。这些细菌可以在消化系统以及人类口腔中找到。目的:本研究的目的是鉴定印度尼西亚年轻人唾液中的罗伊氏乳杆菌。方法:从18-24岁的印度尼西亚受试者中收集了40份唾液样本。进行DNA提取,然后使用PCR完成罗伊氏乳杆菌的鉴定。六名受试者显示阳性结果。阳性样品在Rogosa琼脂中于37°C厌氧培养24小时。在进行DNA提取和PCR鉴定之前,将几个单个菌落分别在肉汤培养基中进一步培养。选择四个最厚的条带用于DNA测序。结果:使用BLAST进行的分析表明,从印尼唾液分离物中获得的两个罗伊氏乳杆菌菌株的值分别为96%(分离物3.11)和95%(分离物5.14)。这证实了基于平均核苷酸同一性(ANI)的新菌株的存在。 3.11和5.14的分离株已分别在DDBJ / EMBL / GenBank注册,登录号为LC382415?和LC382416。结论:可以从印尼年轻人的唾液中分离出罗伊氏乳杆菌新菌株。为了更好地了解这些新的罗伊氏乳杆菌菌株,需要进行涉及生化测试和表型分析的进一步研究。

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