Potential of Rutin and Vildagliptin Combination against Alloxan Induced Diabetic Nephropathy in Mice

摘要

Objective: The aim of present study was to explore antidiabetic and antioxidant actions of rutin, vildagliptin and their combination in the kidney of alloxan induced (150 mg/kg alloxan monohydrate in normal saline injected by intraperitoneal route) diabetic mice. Methods: Animals were allocated into five groups (each group of six mice, n=6) as: i. control group treated with carboxymethyl cellulose (0.5 %, o.d.), ii. a diabetic control group treated with carboxymethyl cellulose (0.5 %, o.d.), iii. diabetic mice treated with rutin (400 mg/kg, o.d.), iv. diabetic mice treated with vildagliptin (30 mg/kg, o.d.) and v. diabetic mice treated with combination of rutin (400 mg/kg, o.d.) and vildagliptin (30 mg/kg, o.d.). The level of antioxidant enzymes such as lipid peroxidase (LPO), catalase (CAT), superoxide dismutase (SOD) and non enzymatic level of reduced glutathione (GSH) was measured in plasma. Similarly level of protein, albumin, creatinine and blood urea nitrogen (BUN) was measured alongwith urinary output and urinary albumin level before as well as after treatment with drugs. Results: The results obtained after treatment with combination of rutin and vildagliptin were more efficient than those obtained from treatment with either rutin or vildagliptin. Likewise histological damage to glomeruli and tubule (hypertrophy) due to diabetic nephropathy was also improved with combination. Conclusion: Our results suggest that the combination of rutin and vildagliptin is more effective than either of the two drugs alone and can be another effective alternative therapeutic for diabetic nephropathy.