METHOD AND KIT FOR EVALUATION OF SUBSTANCE, METHOD FOR SCREENING OF SUBSTANCE, AND METHOD AND KIT FOR DETERMINATION OF PRESENCE OF OCCURRENCE OF DIABETIC NEPHROPATHY OR POTENTIAL RISK OF DIABETIC NEPHROPATHY

摘要

The object aims to identify a novel biomarker that reflects the potential risk of diabetic nephropathy and provide a series of techniques utilizing the biomarker. A substance to be tested is ingested by an animal which has developed diabetic nephropathy or an animal which has a high potential risk of diabetic nephropathy. The concentration of at least one of 8 types of biomarkers in a body fluid from the animal is compared with a reference value to evaluate the effect of ameliorating diabetic nephropathy or the effect of reducing the potential risk of diabetic nephropathy of the substance. Alternatively, a marker substance may be captured by a carrier having, immobilized thereon, a substance having an affinity for the marker substance to calculate the concentration of the marker substance in a body fluid. Also disclosed is a method for screening for a substance by utilizing the above-mentioned evaluation method. Further disclosed is a method for determining the presence or absence of the occurrence of diabetic nephropathy or the potential risk of diabetic nephropathy by using the marker substance as a measure.