Validation of an analytical methodology for determination of tetracyclines residues in honey by UPLC-MS/MS detection

摘要

An analytical method for the quantification of six common tetracyclines and derivatives thereof including tetracycline, 4-epitetracycline, 4-epioxytetracycline, oxytetracycline, 4-epichlortetracycline, chlortetracycline and doxycycline in honey has been validated. The method involved sample preparation in Na₂EDTA- McIlvaine buffer, solid phase extraction and quantification by UPLC-MS. A linear proportionality between chromatogram area and analyte concentration (R2