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Identification of the aroma-active compounds in Longjing tea characterized by odor activity value, gas chromatography- olfactometry, and aroma recombination

机译:通过气味活性值,气相色谱-嗅觉法和香气重组技术鉴定龙井茶中的香气活性化合物

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Volatile compounds in the three Longjing tea were analyzed by gas chromatography–mass spectrometry with method of solid phase microextraction–gas chromatography. Furthermore, aroma-active compounds of three Longjing tea were analyzed by gas chromatography–olfactometry and odor activity value. However, the kinds of aroma-active compounds identified from those two methods were different. The results showed that dihydroactinidiolide (aroma intensity = 9.1–9.2), (Z )-nerolidol (aroma intensity = 8.4–8.8), benzyl alcohol (aroma intensity = 8.1–8.4), and epoxylinalool (aroma intensity = 8.1–8.5) presented the highest aroma intensity in three green tea samples. While geraniol (422.4–2508.3), epoxylinalool (601.5–1033.9), (Z )-nerolidol (235.5–559.3), 2-ethyl-3,5-dimethylpyrazine (328.0–420.6), β-ionone (227.7–387.3) presented higher odor activity values than other compounds. Thus, this study uses recombination aroma models to identify selected important aroma-active compounds from gas chromatography–olfactometry and odor activity value and verifies their effectiveness to identify the aroma profile characterization of three Longjing tea samples using electronic nose analysis and sensory evaluation. The radar plot of electronic nose analysis tallied with sensory evaluation indicated that the odor activity value–based aroma models were more similar to the original Longjing tea aroma than gas chromatography–olfactometry–based models.
机译:采用固相微萃取-气相色谱法通过气相色谱-质谱法分析了三种龙井茶中的挥发性化合物。此外,通过气相色谱-嗅觉法和气味活性值分析了三种龙井茶的香气活性成分。但是,从这两种方法鉴定出的芳香活性化合物的种类是不同的。结果表明,二氢放线菌二醇(香气强度= 9.1–9.2),(iZ)-神经节醇(香气强度= 8.4–8.8),苯甲醇(香气强度= 8.1–8.4)和环氧芳樟醇(香气强度= 8.1–8.4) 8.5)在三个绿茶样品中呈现出最高的香气强度。而香叶醇(422.4–2508.3),环氧芳樟醇(601.5–1033.9),(iZ)-橙花醇(235.5–559.3),2-乙基-3,5-二甲基吡嗪(328.0–420.6),β-紫罗兰酮(227.7– 387.3)具有比其他化合物更高的气味活性值。因此,本研究使用重组香气模型从气相色谱-嗅觉测定法和气味活性值中鉴定出了重要的香气活性化合物,并通过电子鼻分析和感官评估验证了其鉴定三份龙井茶样品香气特征的有效性。与感官评估相符的电子鼻分析雷达图表明,基于气味活性值的香气模型比基于气相色谱-嗅觉法的模型更类似于原始的龙井茶香气。

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