OPTIMIZATION OF BACTERIOCIN PRODUCTION IN PEDIOCOCCUS ACIDILACTICI BA28 USING RESPONSE SURFACE METHODOLOGY

摘要

To enhance production of pediocin BA28, produced by Pediococcus acidilactici, cultivation conditions and medium composition were optimized using response surface methodology (RSM). The Plackett Burman experimental design was effective in searching for the signific ant variables that influence bacteriocin production. From PB Design, three factors peptone, beef extract and initial pH were found to be significant factors and had positive effect on bacteriocin production. The effects of three main factors on bacteriocin production were further investigated by central composite design (CCD). RSM revealed that the maximum bacteriocin production was achieved at peptone concentration of 5% w/v, beef extract concentration of 5% w/v and an initial pH of 6.0. After RSM, the titer of bacteriocin was all most same as obtained in MRS medium which is much costlier than the designed media. Bacteriocin production in a cost effective medium might facilitate industrial scale production of lactic acid bacteriocin and their use as a natural food biopreservative