Hypoglycemic Effect of Analog Rice Made from Modified Cassava Flour (Mocaf), Arrowroot Flour and Kidney Bean Flour on STZ-NA Induced Diabetic Rats

摘要

Background and Objective: Analog rice made from modified cassava flour (mocaf), arrowroot and kidney beans contains high level of dietary fiber and resistant starch potentially consumed as functional food, particularly for diabetes mellitus. However, its hypoglycemic property has not been comprehensively investigated. After previous research on analog rice made from mocaf and kidney beans flour as protein source, arrowroot flour was added to the formulation due to its hypoglycemic effect. The aim of the study was to evaluate hypoglycemic effect of analog rice made from mocaf, arrow root and kidney beans on Streptozotocin-Nicotinamide (STZ-NA) induced diabetic rats . Materials and Methods: Twenty four male Wistar rats were randomly divided into 4 groups, 6 rats each, healthy rats fed with standard feed (H) and three diabetic groups, respectively fed with standard feed (DM), rice variety C4 and mocaf, arrowroot and kidney beans based analog rice (AR). Results: During 4 weeks intervention, feed consumption, body weight and blood glucose level were measured once a week. The results indicated that diabetic rats fed with analog rice (AR)had the highest blood glucose level reduction (55.07%), significantly higher than C4 (18.91%). Total Short Chain Fatty Acids (SCFA) concentration of groups fed with analog rice and rice was 53.96 and 50.76 mmol L^?1, respectively. AR group also had higher Langerhans islets of 10.60 than C4 group of 6.80. The analog rice treatment decreased the blood glucose level. Conclusion: The results indicated that RS and dietary fiber was responsible to glucose reduction effect by analog rice diet through SCFA as resistant starch fermentation product in colon.