TRANSIENT EXPRESSION IN TOBACCO BRIGHT YELLOW 2 CELLS AND POLLEN GRAINS:A FAST, EFFICIENT AND RELIABLE SYSTEM FOR FUNCTIONAL PROMOTER ANALYSIS OF PLANT GENES

摘要

Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruitedtranscription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength andregulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficientand reliable method for testing basal promoter activity and identifying core sequences within its pollen specificelements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUSassay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in bothsystems.