A Fast and Cost-Effective Detection of Melamine by Surface Enhanced Raman Spectroscopy Using a Novel Hydrogen Bonding-Assisted Supramolecular Matrix and Gold-Coated Magnetic Nanoparticles

摘要

A fast and cost-effective melamine detection approach has been developed based on surface enhanced Raman spectroscopy (SERS) using a novel hydrogen bonding-assisted supramolecular matrix. The detection utilizes Fe 3 O 4 /Au magnetic nanoparticles coated with 5-aminoorotic acid (AOA) as a SERS active substrate (Fe 3 O 4 /Au–AOA), and Rhodamine B (RhB) conjugated AOA as a Raman reporter (AOA–RhB). Upon mixing the reagents with melamine, a supramolecular complex [Fe 3 O 4 /Au–AOA???melamine???AOA–RhB] was formed due to the strong multiple hydrogen bonding interactions between AOA and melamine. The complex was separated and concentrated to a pellet by an external magnet and used as a supramolecular matrix for the melamine detection. Laser excitation of the complex pellet produced a strong SERS signal diagnostic for RhB. The logarithmic intensity of the characteristic RhB peaks was found to be proportional to the concentration of melamine with a limit of detection of 2.5 μg/mL and a detection linearity range of 2.5~15.0 μg/mL in milk. As Fe 3 O 4 nanoparticles and AOA are thousands of times less expensive than the monoclonal antibody used in a traditional sandwich immunoassay, the current assay drastically cut down the cost of melamine detection. The current approach affords promise as a biosensor platform that cuts down sample pre-treatment steps and measurement expense.