Microbial transformation of neomycin by a mutant of neomycin-producing Streptomyces fradiae

摘要

Utilizing a mutant of neomycin-producing?Streptomyces fradiae?mutagenized with neutron radiation, biotransformation of neomycin into modified compounds was studied. The biotransformation products were isolated by ion exchange chromatography and monitored by thin layer chromatography bioautography (TLCB). Antibacterial activity of biotransformation products against ten species of bacteria including four plant pathogens was tested qualitatively by TLCB and detected quantitatively by Oxford cup method. The minimal inhibitory concentration (MIC) of biotransformation products was tested by agar diffusion method. Three isolated transformation products had obvious antibacterial activity againstStaphylococcus aureus,?Bacillus subtilis,?Proteus vulgaris?and?Pseudomonas solanacarum. At the concentration of 50 ?g/ml, the transformation product X had a similar antibacterial effect with neomycin but the transformation product Y and Z showed a decreased effect compared to neomycin except for?P. vulgaris?and?P. solanacarum. However, the results from MIC analysis demonstrated that only the transformation product X maintained the same inhibitory effect with neomycin.